Aurintricarboxylic Acid/RNA

Luis Destefano Beltran Luis_Destefano at cornell.edu
Mon Nov 6 01:20:08 EST 1995


Dear netters:

I am isolating total RNA from apple fruits. The extraction buffer 
includes Aurintricarboxylic Acid (ATA). The final total RNA pellet is 
pink. The red book says that this is normal since ATA binds RNA (?). I 
am planning to go on with mRNA isolation and subsequently with cDNA 
library construction My question is: will ATA affect these two steps, if 
yes, how? and what can I do to prevent it? Please answer to 
Luis_Destefano at cornell.edu

Thanks in advance for your help

Luis Destefano





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