hplc of plasmids/small DNA fragments?

Michael Szardenings msz at bio.embnet.se
Mon Nov 6 12:57:46 EST 1995


In article <stephan.witte-311095095102 at marvin.biologie.uni-konstanz.de>,
stephan.witte at uni-konstanz.de (Stephan Witte) wrote:

> Hi,
> 
> has anyone ever tried to purify plasmids/ezymatic digests of plasmids using
> hplc?
> What type of column and what type of solvents?
> 
> Thanks a lot for hints,
> 
> Stephan

As someone mentioned above Pharmacia's MonoQ may be used for it. I
actually remember some chromatogram in a booklet for ionexchangers from
this company, that showed a plasmid digest. Perhaps ask the Pharmacia
people in Freiburg about it.
(Disclaimer:I am not acquainted/involved/payed or whatsoever by the company)

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