BIIIIG problem with top-agarose

[Stephen Mamber]

In article <47jn9q$8hn at news.cc.utah.edu>, zinc at zifi.genetics.utah.edu (zinc) says:
>Hilde Nilsen  <hilden at unigen.unit.no> wrote:
>>I am screening a genomic lambda libary. I am using host cells without selection.
>>The problem is that as I am transferring the plaques to Hybond N+ membranes, 
>>the top-agarose adheres to the membrane thereby ruining everything.
>>I suspect this to happen because of moisture between the top-agarose and the medium.

You might want to increase your agarose concentration 0.1-0.2% from
whatever you are using (I suspect 0.6%). 


SWM

mamber at synapse.bms.com