Michaelis constants of polymerases ?

Peng, Hsien-wei b1205116 at cc.ntu.edu.tw
Mon Nov 6 09:31:20 EST 1995


Dear Netters,

  Is there any manual or review article listing the Michaelis constant (Km)

of DNA polymerases such as Klenow fragment and Taq polymerase?  I need

these data beacuse I am finding a minimal concentration of dNTPs used in 

the DNA labeling reactions.  Thanks a lot in advance!


===========================================================================
Hsien-wei Peng

Inst. of Biomedical Sci., Acad. Sinica., Taiwan
E-mail:b1205116 at cc.ntu.edu.tw




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