>>In article <47fj07$n4v at due.unit.no>,
>Hilde Nilsen <hilden at unigen.unit.no> wrote:
>>>>I am screening a genomic lambda libary. I am using host cells without
>>The problem is that as I am transferring the plaques to Hybond N+ membranes,
>>the top-agarose adheres to the membrane thereby ruining everything.
>>I suspect this to happen because of moisture between the top-agarose and the
>>>>So, what can I do to prevent this? I am cooling the medium to 50 deg. celsius
>>preparing the plates so too hot medium should not be the problem.
>>i have two suggestions for you.
>>1) try to use plates that have been 'cured' for a day or two so that
>they are not to wet when you apply the top agar.
>>2) this is what i usually do to prevent the top agar from lifting off.
>i just cool the plates to 4 deg C after the plaques have grown and i
>have not had any problems with it lifting off when i do this.
>Maniatis also used to suggest using Agarose instead of agar to make your top
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