covalent protein-plastic linkage

Keld Sorensen KeldS at uic.edu
Mon Nov 6 14:03:45 EST 1995


"Covalent" avidin & streptavidin on ELISA plates seems impossible in
theory and very do-able, i.e. it works in practical applications.
Since avidin/SA is a tetramer which can not possible have all
4 subunits coupled to the plate, it really does not matter much
that say 1 unit is covalently coupled - the other 3 may come off.
In practical usage, the commercial SA plates (Pierce etc) do
not leach avidin/Streptavidin - i.e. for all practical purposes
it is covalent. You could coat into plates that react with the
protein covalently, but as I said, it is probably not worth the
extra expense.
Keld.





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