>dlburg at umich.edu (Dan Burgess) writes:
> Does anyone out there have a good idea of which Reverse Transcriptase
> (RT) has the highest activity under mammalian cellular conditions
> (37 degrees C, ionic conditions, etc.)? I'm looking for one which is
> cloned, and preferably if the gene is available for non-commercial
> basic research use. Please reply either here or to: dlburg at umich.edu> Many thanks in advance for your help.
>> Dan Burgess
> U of Michigan, Dept. of Human Genetics
> Ann Arbor, MI.
>>http://www.umich.edu/~dlburg/homepage.html>dlburg at umich.edu>>>>>MMLV reverse transcriptase is generally a good choice, if 37 C is a must for your reaction. It is often used interchangeably with the
same reaction buffers that are supplied/used for reverse transcription with AMV reverse transcriptase. If you are looking for a good
kit in which these enzymes can be used interchangeably, please let me know at the e-mail address below and I'll let you which I use
workshop at dnatech.com