High Resolution mini-gels?
S.K.Green
S.K.Green at shef.ac.uk
Thu Nov 2 09:29:36 EST 1995
Dear Petros,
An afterthought, you might be better end-labelling your fragments and
running on a sequencing gel.
The other thing you can try is Nusieve agarose(FMC). It really does
depend on the sizes you want to resolve.
Good luck
Sue GPetros
, BKXO at musicb.mcgill.ca wrote:
>I would like to differentiate by gel electrophoresis between DNA
>fragments differing in size by as little as a few base pairs. The size of
>the DNA fragments are between 500-900bp and some differ by as little
>as 5bp.
>
>Can anyone suggest a high resolution mini-gel system that might
>accomplish separation of DNA fragments differing in size by as little
>as 5bp?
>
>Petros
>BKXO at musicb.mcgill.ca
>
reen
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