High Resolution mini-gels?

S.K.Green S.K.Green at shef.ac.uk
Thu Nov 2 09:29:36 EST 1995


Dear Petros,

An afterthought, you might be better end-labelling your fragments and 
running on a sequencing gel.  

The other thing you can try is Nusieve agarose(FMC).  It really does 
depend on the sizes you want to resolve.

Good luck

Sue GPetros
, BKXO at musicb.mcgill.ca wrote:
>I would like to differentiate by gel electrophoresis between DNA 
>fragments differing in size by as little as a few base pairs.  The size of 
>the DNA fragments are between 500-900bp and some differ by as little 
>as 5bp.
>
>Can anyone suggest a high resolution mini-gel system that might
>accomplish separation of DNA fragments differing in size by as little 
>as 5bp?
>
>Petros
>BKXO at musicb.mcgill.ca
>
reen





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