I routinely use an acrylamide vertical gel system (Bio-rad mini protean
for example). Use acrylamide, TBE and water to make up gel and set with
TEMED and ammonium persulphate. You can separate DNA fragments of
similar sizes depending on the acrylamide concentration you use. I use
9% to separate fragments of 200-300bp, you might need to lower or
increase the acrylamide, depending on your separation.
Sue Green, Ph.D.
University of Sheffield, UKPetros, BKXO at musicb.mcgill.ca wrote:
>I would like to differentiate by gel electrophoresis between DNA
>fragments differing in size by as little as a few base pairs. The size of
>the DNA fragments are between 500-900bp and some differ by as little
>>Can anyone suggest a high resolution mini-gel system that might
>accomplish separation of DNA fragments differing in size by as little
>BKXO at musicb.mcgill.ca>