High Resolution mini-gels?

Daniel Kim dkim at nmsu.edu
Wed Nov 1 09:37:26 EST 1995


In article <475mej$r6u at sifon.cc.mcgill.ca> Petros, BKXO at musicb.mcgill.ca writes:
>I would like to differentiate by gel electrophoresis between DNA 
>fragments differing in size by as little as a few base pairs.  The size of 
>the DNA fragments are between 500-900bp and some differ by as little 
>as 5bp.
>
>Can anyone suggest a high resolution mini-gel system that might
>accomplish separation of DNA fragments differing in size by as little 
>as 5bp?
>
>Petros
>BKXO at musicb.mcgill.ca
>


I believe the Hoefer Tall Mighty Small rig is capable of single base 
resolution, and there is a paper in which it was used to sequence DNA.

Call them for details.  If you need it, I could give you the citation.

Daniel Kim
dkim at nmsu.edu




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