Protocol for Immunostaining tissue sections for B-galactosidase
adj8n at avery.med.virginia.edu
Tue Nov 7 10:30:16 EST 1995
We are looking for a protocol to immunostain for B-gal in
sectioned tissues of embryos. The original embryos were fixed in
paraformaldehyde for 48hrs, then whole-mount stained for B-gal. We got
great staining for B-gal, but unfortunately when the tissues were
sectioned into 10um thick paraffin sections, the blue stain was too light
to be visible anymore. We would like to use the Sigma (or other)
antibody to B-gal to immunostain the protein, since we already know
approximately where it is located, but cannot see it specifically.
So does anyone have a protocol, knowledge, suggestions, caveats,
etc., about trying this. All help would be greatly appreciated.
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