Better denaturant than SDS?

Christian Belanger chrisbel at eukaryota
Tue Nov 7 09:35:07 EST 1995


Hi netters,

I want to stop enzymatic reaction without using heat.  I work under in situ
condition with raw material.  Heating extract a lot of unwanted stuff.  I
used SDS as denaturing agent but it's a bit too foamy.  Any suggestion on
better denaturing agent?  Any other idea on stopping enzymatic reaction
under in situ conditions with raw material?

E-mail me your answers.

Thank's.

Christian Belanger
c_belanger2 at qc.dfo.ca



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