Salt-free DNA precipitation?
diabetes at lrz.uni-muenchen.de
Mon Nov 6 19:04:31 EST 1995
I'm new to PCR, so let me ask a question, please: Is this a common
protocol or is it home-brew? If you have one, please give me
Have a nice day (PCR product ;-)
Koen De Smet <kds30> wrote:
>I may be ableto help you to get halfway: this technique still uses salt in the precipitation, but no alcohol, while the wash (as uss=
>ual) does not contain salts).
>I routinely precipitate PCR products by adding 1/10 V of 3M sodiumacetate pH 5.2 and 1V of 30% PEG-6000. Leave for 15 minutes at roo=
>m temperature and spin. Wash with 70% ethanol.
>This precipitates the PCR product, but not the oligos, so the DNA can be used for sequencing.
>Koen De Smet
Chemist, PhD Student
Institute for Diabetes Reserch
Koelner Platz 1
Phone +49 (89) 30 79 31 24
Fax +49 (89) 30 81 733
email u7k0201 at sunmail.lrz-muenchen.de
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