DNA extraction of 140 kb piece from agarose

[Phil Terman]

bm335 at FreeNet.Carleton.CA (Christina Chan) wrote:
>I have been having difficulty in isolating a 140 kb piece of DNA
>from a TBE agarose (1% SeaKem GTG) plug.  The plug contains
>~500 ng of DNA.  I have tried the Gene Clean II kit but was un-
>successful.
>
>Any suggestions?
**********

Have you tried the Ultrafree MC 0.45um device by Millipore?
They have a method for eluting DNA from agarose in just five minutes.  Works for me.