DNA extraction of 140 kb piece from agarose
gambler at cts.com
Tue Nov 7 20:50:24 EST 1995
bm335 at FreeNet.Carleton.CA (Christina Chan) wrote:
>I have been having difficulty in isolating a 140 kb piece of DNA
>from a TBE agarose (1% SeaKem GTG) plug. The plug contains
>~500 ng of DNA. I have tried the Gene Clean II kit but was un-
Have you tried the Ultrafree MC 0.45um device by Millipore?
They have a method for eluting DNA from agarose in just five minutes. Works for me.
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