High Resolution mini-gels?

Michael Myers myersm at rockvax.rockefeller.edu
Tue Nov 7 22:45:38 EST 1995


In article <gustilo-0511951658150001 at ts11-44.upenn.edu>,
gustilo at pobox.upenn.edu wrote:

>In article <475mej$r6u at sifon.cc.mcgill.ca>, Petros, BKXO at musicb.mcgill.ca
wrote:
>
>> I would like to differentiate by gel electrophoresis between DNA 
>> fragments differing in size by as little as a few base pairs.  The size of 
>> the DNA fragments are between 500-900bp and some differ by as little 
>> as 5bp.
>> 
As others have said, acrylamide would be best. Metaphor agarose from FMC
was also mentioned, and I can say from experience that it works very well.

Michael Myers
Laboratory of Genetics
The Rockefeller University
212-327-8233  (-7420 fax)
myersm at rockvax.rockefeller.edu



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