In article <47agm2$6qc at hippo.shef.ac.uk>, M.C.Behrendt at sheffield.ac.uk wrote:
> Please could any netters in the know tell me if ligation of PCR products
> into a T-vector requires any special conditions and if these alter from
> a normal ligation?
>the only special condition is that you don't need to gel purify the
product first. just take an aliquot (with the TA vector from Invitrogen i
always use 2ul of a 25-50ul reaction) of the product, mix it with the
vector, buffer and ligase and go for it.
Eric C. Anderson
Cell Biology and Genetics
Memorial Sloan-Kettering Cancer Center
1275 York Ave., Box 470
New York, NY 10028
e-anderson at ski.mskcc.org