Help with T-vector

Eric C. Anderson e-anderson at ski.mskcc.org
Wed Nov 8 08:44:30 EST 1995


In article <47agm2$6qc at hippo.shef.ac.uk>, M.C.Behrendt at sheffield.ac.uk wrote:

> Please could any netters in the know tell me if ligation of PCR products
> into a T-vector requires any special conditions and if these alter from
> a normal ligation?
> 
the only special condition is that you don't need to gel purify the
product first.  just take an aliquot (with the TA vector from Invitrogen i
always use 2ul of a 25-50ul reaction) of the product, mix it with the
vector, buffer and ligase and go for it.

eric

-- 
Eric C. Anderson
Cell Biology and Genetics
Memorial Sloan-Kettering Cancer Center
Sloan-Kettering Institute
1275 York Ave., Box 470
New York, NY  10028
Phone: (212)639-2977
Fax:   (212)717-3298
e-anderson at ski.mskcc.org



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