question about minimum overlapping bases for oligomer annealing
ut
ut
Thu Nov 9 04:18:54 EST 1995
I want to generate a fragment of 70bp double strand DNA. The
stratage I choose is as followes:
Synthesize two oligomers as short as possible with their 3' end
complementary with each other. Then anneal these two oligomers by
means of those 3'-complementary parts. The rest of the gap will be
filled by klenow. My question is how many complementary bases that
I should have at least on the two oligomers are sufficient to allow
them anneal well and successfully for klenow filling in
subsequently? Is 6 bases long enough? Your suggestion is appreciated
very much. Please send you suggestion to:
nianhua at utmmg.med.uth.tmc.edu
More information about the Methods
mailing list