proofreading thermostable polymerases: Pfu vs. Pwo

N. van Belzen at HEMA BELZEN at hema.fgg.eur.nl
Wed Nov 8 04:58:22 EST 1995


Hi netters,

I need a high fidelity thermostable polymerase.

According to the literature, Pfu (Stratagene) is approx. 10-fold more 
accurate than Taq (e.g. Flaman et al. 1994, Nucl. Acids Res. 22:3259). 
According to the info of Boehringer, Pwo also beats Taq 10 to 1, but I have 
seen no data on this from independent labs.

Anybody any suggestions/experiences with these (and other) proofreading 
enzymes?

Best regards, Nico van Belzen



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