DEPC-treating buffers, salt solutions vs filtering
howe at DARWIN.UCSC.EDU
Wed Nov 8 19:16:13 EST 1995
What's the concensus on DEPC-treating buffers (PIPES, HEPES, Tris, etc)
or salt solutions (NaCl, MgSO4, MgCl2, Mn++, Zn++ etc) vs filter
sterilization vs autoclaving for removal of RNases? Can I treat
previously made solutions, or do I need to make them in DEPC-treated
water over again?
Any info regarding how to get rid of these pesky buggers would be
Thanks. Responses can be sent to me at howe at biology.ucsc.edu or to the
net if there's a general interest.
"The onions expressed here are my own"
Sherif, keep this under your hat, OK?
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