DEPC-treating buffers, salt solutions vs filtering

Ken Howe howe at DARWIN.UCSC.EDU
Wed Nov 8 19:16:13 EST 1995


Dear Netters,
What's the concensus on DEPC-treating buffers (PIPES, HEPES, Tris, etc) 
or salt solutions (NaCl, MgSO4, MgCl2, Mn++, Zn++ etc) vs filter 
sterilization vs autoclaving for removal of RNases?  Can I treat 
previously made solutions, or do I need to make them in DEPC-treated 
water over again?

Any info regarding how to get rid of these pesky buggers would be 
appreciated.

Thanks.  Responses can be sent to me at howe at biology.ucsc.edu or to the 
net if there's a general interest.

Ken Howe
"The onions expressed here are my own"

Sherif, keep this under your hat, OK?



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