>>> On Wed, 8 Nov 1995 17:20:45 +0000 (GMT), d.coates at leeds.ac.uk (David Coates) said:
David> Has anyone come across/used E.coli XL1 Red for mutagenesis? Any
David> information gratefully received.
I tried using it to mutate a lactmase carried in an M13 phage vector.
Two rounds of overnight growth in Xl1red, followed by propagation in
Xl1-blue with B-lactam antibiotics to select active enzymes. The 6
or 8 clones sequenced had no mutations. Boo.
Nathan O. Siemers, Ph.D.
Bristol-Myers Squibb Pharmaceutical Research Institute
3005 First Avenue, Seattle, WA 98121
(206) 727-3741 siemers at bms.com