proofreading thermostable polymerases: Pfu vs. Pwo

george vandermerwe gvandermerwe at utmem1.utmem.edu
Wed Nov 8 04:17:27 EST 1995


In article <BELZEN.26.815824702 at hema.fgg.eur.nl>, BELZEN at hema.fgg.eur.nl
(N. van Belzen @ HEMA) wrote:

> Hi netters,
> 
> I need a high fidelity thermostable polymerase.
> 
> According to the literature, Pfu (Stratagene) is approx. 10-fold more 
> accurate than Taq (e.g. Flaman et al. 1994, Nucl. Acids Res. 22:3259). 
> According to the info of Boehringer, Pwo also beats Taq 10 to 1, but I have 
> seen no data on this from independent labs.
> 
> Anybody any suggestions/experiences with these (and other) proofreading 
> enzymes?
> 
I have used both these enzymes in PCR reactions and found them to be very
reliable.  Think you should be safe using any one of these.

Regards
George

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