Design of dye terminator primers
bbur at wpo.nerc.ac.uk
Thu Nov 9 11:34:46 EST 1995
(Dr. J.P. Clewley) writes:
>Has anyone any hints or tips for the design of primers specifically
>for cycle sequencing with an ABI dyedeoxy terminator kit?
>Virus Reference Division
>Central Public Health Laboratory
>61 Colindale Avenue
>London NW9 5HT
>jclewley at hgmp.mrc.ac.uk
I just used my pcr primers initially, then a couple of internal 24-mers, I
didn't do anything 'special', just less than 50% GC and a good 3' end
preferably with a G or C at the end... I think ABI have an information sheet
they'll send you but as I recall it doesn't say anything non-'standard'...
If it's a terminator chemistry does the primer make any difference anyway?
If someone knows otherwise could you post or email their words of wisdom?
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