In article <cliff.18.30A1A025 at molbiol.uct.ac.za>, cliff at molbiol.uct.ac.za wrote:
> Dear All
>> I am searching for a bacterial cloning vector which contains unique XbaI and
> PvuI (or PacI) restriction sites in its polylinker. Any suggestions ??
Try pNEB193 from New England Biolabs (has both PacI and XbaI sites). Since
PvuI site is present in Ap-res gene it will be difficult to find
combination PvuI + XbaI. Moreover, PvuI overhangs are poorly ligated due
to dam methylation in most common cloning strains.