PacI or PvuI cloning vectors

M. Alexeyev malexeyev at biost1.thi.tmc.edu
Thu Nov 9 09:16:54 EST 1995


In article <cliff.18.30A1A025 at molbiol.uct.ac.za>, cliff at molbiol.uct.ac.za wrote:

> Dear All
> 
> I am searching for a bacterial cloning vector which contains unique XbaI and 
> PvuI (or PacI) restriction sites in its polylinker. Any suggestions ??

Try pNEB193 from New England Biolabs (has both PacI and XbaI sites). Since
PvuI site is present in Ap-res gene it will be difficult to find
combination PvuI + XbaI. Moreover, PvuI overhangs are poorly ligated due
to dam methylation in most common cloning strains.

Good luck,
M. Alexeyev



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