In article <9511081935.AA18583 at umailsrv1.UMD.EDU> Lynne_A_WHITEHEAD at UMAIL.UMD.EDU (lw75) writes:
>From: Lynne_A_WHITEHEAD at UMAIL.UMD.EDU (lw75)
>Subject: protein sequencing from a western blot
>Date: 8 Nov 1995 11:37:12 -0800
>I am interested in learning more about protein sequencing from western blots.
>1. is this method reliable? How much protein is required for successful
Yes, the method is reliable. In general about 5 pmole for a N-terminus
sequence of up to 40 aa
>2. can it be done from an SDS-PAGE gel as well as a native PAGE
In theory it can, as long as you are sure that what you get on the native
PAGE is a single species of polypeptide.
>3. does anyone know of any companies with the sequencing apparatus that
will>process the samples for you?
I do know one here in the Netherlands, but
there should be plenty of them in the States.
>Any references would be appreciated!
Good luck Ton