Design of dye terminator primers

John E. Fox altabios at bham.ac.uk
Thu Nov 9 08:33:27 EST 1995


In article <47sn6h$ivh at mserv1.dl.ac.uk>, jclewley at hgmp.mrc.ac.uk (Dr. J.P. Clewley) says:
>
>
>Has anyone any hints or tips for the design of primers specifically
>for cycle sequencing with an ABI dyedeoxy terminator kit?
>
>Jon Clewley
>Virus Reference Division
>Central Public Health Laboratory
>61 Colindale Avenue
>London NW9 5HT
>United Kingdom
>
>jclewley at hgmp.mrc.ac.uk


Key things are:- C or G at 3' and a TM of 50-55C.
Beyond that, nothing special at all.

John Fox



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