Looking for E.coli Y1089

Anton Scott Goustin asg at cmb.biosci.wayne.edu
Thu Nov 9 20:42:40 EST 1995


nam at biochem.kaist.ac.kr (Sang-Chul Nam) wrote:
>Could anybody send me E. coli Y1089?
>Or give me some information how can get this strain.
>
>--
>Sang-Chul Nam (nam at biochem.kaist.ac.kr) 
>Dept. of Biological Sciences, KAIST
>373-1 Kusong-dong, Yusong-gu 
>Taejon 305-701, South Korea

Promega sells lambda gt11 cloning kit as well as the strain Y1089.  
Check out their WWW page:  
http://www.promega.com:80/tbs/TB006/tb006.html#ii

Where you will read about:
Y1089 (1): 

The strain Y1089 is used primarily for the generation of preparative 
amounts of fusion protein (see
Section VIII). This is possible because of a mutation which enhances the 
frequency of phage
lysogeny (hflA150) and the deltalon mutation which decreases protease 
activity. Like Y1090, this
strain also contains pMC9 (described above). 

Genotype: F´, delta(lacU169), proA+, delta(lon), araD139, strA, hflA150, 
[chr:Tn10(tetr)],
(pMC9). 





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