nam at biochem.kaist.ac.kr (Sang-Chul Nam) wrote:
>Could anybody send me E. coli Y1089?
>Or give me some information how can get this strain.
>Sang-Chul Nam (nam at biochem.kaist.ac.kr)
>Dept. of Biological Sciences, KAIST
>373-1 Kusong-dong, Yusong-gu
>Taejon 305-701, South Korea
Promega sells lambda gt11 cloning kit as well as the strain Y1089.
Check out their WWW page:
Where you will read about:
The strain Y1089 is used primarily for the generation of preparative
amounts of fusion protein (see
Section VIII). This is possible because of a mutation which enhances the
frequency of phage
lysogeny (hflA150) and the deltalon mutation which decreases protease
activity. Like Y1090, this
strain also contains pMC9 (described above).
Genotype: F´, delta(lacU169), proA+, delta(lon), araD139, strA, hflA150,