Do not treat reagents which contain any primary amine group with DEPC (eg. tris buffers). Water used for making up such buffers
can be treated with DEPC and then autoclaved, and then used to make up tris and related buffers, which are then autoclaved a
second time. You should aliquot your reagents so that in the event of nuclease contamination, the reagents you discard will be of
For those reagents which are non-autoclavable, filtering through 0.22 or 0.45 micron nitrocelluose filters is desirable. Of course, the
inclusion of ribonuclease inhibitors, such as VDR or RNasin or related products, may solve some of the problems which you may be
experiencing, presumably with the isolation of RNA.
Source: RNA Methodologies: A Laboratory Guide for Isolation and Characterization. Farrell, 1993. Academic Press.