DEPC

workshop at dnatech.com workshop at dnatech.com
Thu Nov 9 10:20:13 EST 1995


Do not treat reagents which contain any primary amine group with DEPC (eg. tris buffers).   Water used for making up such buffers 
can be treated with DEPC and then autoclaved, and then used to make up tris and related buffers, which are then autoclaved a 
second time.  You should aliquot your reagents so that in the event of nuclease contamination, the reagents you discard will be of 
minimal volume.

For those reagents which are non-autoclavable, filtering through 0.22 or 0.45 micron nitrocelluose filters is desirable.  Of course, the 
inclusion of ribonuclease inhibitors, such as VDR or RNasin or related products, may solve some of the problems which you may be 
experiencing, presumably with the isolation of RNA.

Source: RNA Methodologies: A Laboratory Guide for Isolation and Characterization.  Farrell, 1993.  Academic Press.



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