DNA-Mobility-Shift with Biotin labeled Oligo?
bojar at uni-duesseldorf.de
Thu Nov 9 19:43:09 EST 1995
Does anybody know something about a gel mobility shift assay, whitch uses
I synthesized a dubblestranded oligo of the estrogen response element. The
oligo is labeled on both 5´ ends. Do I have a chance to see a gel mobility
shift, by mixing the oligo with crude or purified estrogen-receptor extracts.
Blotting and Streptavidin-POD assay shall be used for detection.
In the normal procedure always 32P is used.
Who knows a method or protocol that could help me?
Thanks in advance
Onkologie Duesseldorf Germany
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