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DEPC-treating buffers, salt solutions vs filtering

Eric C. Anderson e-anderson at ski.mskcc.org
Thu Nov 9 08:44:09 EST 1995

In article <Pine.SOL.3.91.951108155942.2870A-100000 at darwin.UCSC.EDU>,
howe at DARWIN.UCSC.EDU (Ken Howe) wrote:

> Dear Netters,
> What's the concensus on DEPC-treating buffers (PIPES, HEPES, Tris, etc) 
> or salt solutions (NaCl, MgSO4, MgCl2, Mn++, Zn++ etc) vs filter 
> sterilization vs autoclaving for removal of RNases?  Can I treat 
> previously made solutions, or do I need to make them in DEPC-treated 
> water over again?

one thing to keep in mind is that you can't treat any buffer with a free
amino group (i.e. Tris) with DEPC...best thing that you can do is sterile
filter or autoclave and hope for the best.


Eric C. Anderson
Cell Biology and Genetics
Memorial Sloan-Kettering Cancer Center
Sloan-Kettering Institute
1275 York Ave., Box 470
New York, NY  10028
Phone: (212)639-2977
Fax:   (212)717-3298
e-anderson at ski.mskcc.org

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