E.coli Y1089 vs. 1090 question

Petri Kursula pkursula at cc.oulu.fi
Fri Nov 10 04:39:28 EST 1995


Im going to screen an expression library with antibodies, and I have 
these two strains Y1089 and Y1090. Which would be a better host? Somebody 
has managed to lose the MC9 plasmid from our 1090 strain, so I tried 
Y1089 but it apparently only makes lysogens since there are no plaques. 
the phages are there I checked it with LE392. So... is there a way of 
making Y1089 to produce phage plaques, or should I get a new batch of Y1090?

Pete




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