RNA: Maxam-Gilbert C-Reaktion Problems

Bernd Schu bschu
Fri Nov 10 04:48:09 EST 1995


Hi folks outthere,

I want to use the Maxam Gilbert C-Reaktion to probe RNA-structure.
To do this you have to incubate the RNA with DMS to get the Cytidine 
methylated. Then you perform hydozinolysis. This step will cleave at
C and U (U is cleaved structur-undependend and is not of interest).
When I do this I get only very little scission and the major part of 
the RNA sample remains at the starting point of elektrophoresis.This is
reproduceable. 
I can perform G-reaction under the same conditions and it works perfectly.

Has anyone an idea what happend with this C-reaction ? Has anyone had the
same result.

Please mail answers to Bernhard.schu at physik.uni-ulm.de

Thanks in advance

Bernd (-:




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