Hi folks outthere,
I want to use the Maxam Gilbert C-Reaktion to probe RNA-structure.
To do this you have to incubate the RNA with DMS to get the Cytidine
methylated. Then you perform hydozinolysis. This step will cleave at
C and U (U is cleaved structur-undependend and is not of interest).
When I do this I get only very little scission and the major part of
the RNA sample remains at the starting point of elektrophoresis.This is
I can perform G-reaction under the same conditions and it works perfectly.
Has anyone an idea what happend with this C-reaction ? Has anyone had the
Please mail answers to Bernhard.schu at physik.uni-ulm.de
Thanks in advance