POLYMERASE and TA CLONING SYSTEM CONSTRUCTION

HAMELS Sandrine shamels at biocell.fundp.ac.be
Fri Nov 10 11:01:58 EST 1995


   In order to construct our proper TA cloning system, we have to
introduce an  adenine at the end of the PCR product.

   In our lab, we use the DYNAZYME polymerase (commercialised by
FINNZYMES) and we wonder if this polymerase is able to add a A nucleotide
such as the Taq polymerase do.

   If you have any information about the DYNAZYME polymerisation, please
send us some details.

   Thank you in advance.

-- 
Sandrine HAMELS
Laboratory of Cellular Biochemistry,
Facultes Universitaires ND de la Paix,
61, rue de Bruxelles,
B-5000 Namur (Belgium).
Fax: ++/32/81/72.41.35.
Email: michet at biocell.fundp.ac.be



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