Need fast mini-prep protocols

John R. Thompson jrt at intac.com
Sat Nov 11 14:21:52 EST 1995


lur at duke.usask.ca (Rui Lu) wrote:

>Hi, netters,
>
>	Now I have a large number of colonies to screen for insert.
>Does anyone know any fast mini-prep procedures to yield enough, cut-
>able plasmid DNA?
>	
You could try a whole cell PCR approach.  Use PCR with one primer
specific for the insert, the other primer specific for an adjacent
region on the plasmid.  Alternatively, if the inserts are not too
large you can just  use vector primers flanking the insert site too.
Now, just do whole cell PCR and run a gel to identify insert
containing clones.

Regards,
John R.  Thompson
Merck Research Labs




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