Help with T-vector

Sat Nov 11 13:38:27 EST 1995

In article <47agm2$6qc at>, <M.C.Behrendt at> writes:
> Path:!!!!oleane!!ma!!!!lamarck.sur!!biosci!daresbury!!warwick!
uk!newsmaster at sheffield
> From: M.C.Behrendt at (M.C.Behrendt)
> Newsgroups: bionet.molbio.methds-reagnts
> Subject: Help with T-vector
> Date: 2 Nov 1995 13:22:10 GMT
> Organization: Molecular Biology & Biotechnology, University of Sheffield , UK
> Lines: 7
> Message-ID: <47agm2$6qc at>
> Reply-To: M.C.Behrendt at
> NNTP-Posting-Host:
> X-Newsreader: WinVN 0.92.1
> Please could any netters in the know tell me if ligation of PCR products
> into a T-vector requires any special conditions and if these alter from
> a normal ligation?
> Thanks in advance 
> Mark

Hi Mark,
	Of course you must use a polymerase that adds a A' at the end of the 
strand; but the conditions you should use are normally 12-15 degrees 
centigrades with a T4 ligase for 3-5 hours. That must be enough to get good 
	I hope this is of your interest

More information about the Methods mailing list