DNA purification from Agarose (Sigma)

ANTONIO.ARNAIZ at INM.H12O.ES ANTONIO.ARNAIZ at INM.H12O.ES
Sat Nov 11 13:29:44 EST 1995


In article <45fr29$74p at worak.kaist.ac.kr>, <hbkim at biochem.kaist.ac.kr> writes:
> Path: 
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> From: hbkim at biochem.kaist.ac.kr (Hanbok Kim)
> Newsgroups: bionet.molbio.methds-reagnts
> Subject: DNA purification from Agarose (Sigma)
> Date: 11 Oct 1995 07:17:29 GMT
> Organization: Biochemistry Lab, KAIST
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> 
> For DNA purification, Geneclean II Kit (Bio101) with
> TBE modifier and Sigma Agarose (A9539) was used.
> However subcloning was very hard by this method, suggesting
> some inhibitory effects on ligation by either Agarose 
> or Kit ingredients. Ligation as a control was ok.
> I would appreciate any advices to solve the problem, and
> ingredients of TBE modifier.
> 

Hi kim
	An alternative would be use Nusieve GTG, TEA as buffer, and Sephaglas 
(pharmacia).
	Good luck




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