Yep, I use the Dynazyme enzyme as well. They say you
need at least a 10 min soak at 72deg.C at the end of your cycling to
add the A residue. I use a 30 min. soak just to make sure and it
works fine for me. Hope that helps.
Dr. Simon Dawson TEL:+44 (0)115 9249924 ex. 44789
Dept. of Biochemistry FAX:+44 (0)115 9422225
Queens Medical Centre Email:Simon.Dawson at .nott.ac.uk
U.K. "Back off man, I'm a scientist!" - Bill