Northern Transfer buffer?

Z. Jeffrey Chen chen_j at BIODEC.WUSTL.EDU
Sun Nov 12 01:07:29 EST 1995


> On Fri, 10 Nov 1995, Jeffrey Upperman wrote:
> 
> > i have a simple question.  someone on our floor claims that you can use 
> > 1X TAE as a transfer buffer for northern blot transfer in place of 10 or 
> > 20X SSC.  i say no...they say yes...comments?
> 
 Jeff:

I used the TE solution for RNA transfer all the time. It works just fine. In 
fact, I can re-use the bolots a couple of times by boiling the blots in 
DEPC-H2O. Good luck.

Jeff.

Z. Jeffrey Chen
Biology Department
Box 1137, One Brookings Drive
Washington University
St. Louis, MO 63130
Phone (314) 935-8529
Fax (314) 935-4432




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