Northern Transfer buffer?

[Mark S. Whitsitt]

In article <upperman.816035285 at njmsa>,
   upperman at njmsa.UMDNJ.EDU (Jeffrey Upperman) wrote:
>
>i have a simple question.  someone on our floor claims that you can 
use 
>1X TAE as a transfer buffer for northern blot transfer in place of 
10 or 
>20X SSC.  i say no...they say yes...comments?

I regularly use 25 mM sodium phosphate buffer, pH 7.5 I believe.. 
I'll have to check the exact pH.

Mark

Mark S. Whitsitt, N5RJF        Texas A&M University, Dept of Biochemistry
Internet:  mwhitsitt at tamu.edu             College Station, Tx. 77843-2128
WWW URL: http://tam2000.tamu.edu/~markw                    (409) 845-0832