Northern Transfer buffer?
Mark S. Whitsitt
mwhitsitt at tamu.edu
Sun Nov 12 01:27:06 EST 1995
In article <upperman.816035285 at njmsa>,
upperman at njmsa.UMDNJ.EDU (Jeffrey Upperman) wrote:
>i have a simple question. someone on our floor claims that you can
>1X TAE as a transfer buffer for northern blot transfer in place of
>20X SSC. i say no...they say yes...comments?
I regularly use 25 mM sodium phosphate buffer, pH 7.5 I believe..
I'll have to check the exact pH.
Mark S. Whitsitt, N5RJF Texas A&M University, Dept of Biochemistry
Internet: mwhitsitt at tamu.edu College Station, Tx. 77843-2128
WWW URL: http://tam2000.tamu.edu/~markw (409) 845-0832
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