Staining membrane in Southern blotting

Hakan Janson Hakan.Janson at mikrobiol.mas.lu.se
Mon Nov 13 03:29:54 EST 1995


hopkins at surgadmin.surgery.wisc.edu (W.J. Hopkins) wrote:

>   Is it possible to reversibly stain a membrane after transfer of
>restriction fragments from a gel?  This can be done with proteins
>transferred to nitrocellulose in W. blots, so I was wondering if an
>analagous procedure could be used in to monitor DNA transfer.

One way  is to simply put the membrane under long wave UV-light after
transfer. The method of course requires that you have stained your DNA
fragments with ethidium bromide. The method is relatively crude but it
nevertheless gives you a way to see whether the DNA transfer was
successfuk or not. 




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