luciferase gene activity

tsang chiu suet margaret csmtsang at hkusua.hku.hk
Mon Nov 13 03:38:41 EST 1995


HI Netters,
we are trying to clone a promoter fragment into promega'a 
pGL3 luciferase reporter. Is anyone know whether the 
luciferase will still be active if a fusion enzyme is 
formed? ie, If there is appro. 20 amino acid fuse with the
lucifase in  the 5' end? 
We know that it would not harm B-galactosidase but for 
luciferase we are not sure.

Thanks for your help

Ben Ko
H8913564 at hkucc.hku.hk





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