we are trying to clone a promoter fragment into promega'a
pGL3 luciferase reporter. Is anyone know whether the
luciferase will still be active if a fusion enzyme is
formed? ie, If there is appro. 20 amino acid fuse with the
lucifase in the 5' end?
We know that it would not harm B-galactosidase but for
luciferase we are not sure.
Thanks for your help
H8913564 at hkucc.hku.hk