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Peptide biotinylation

Michael Daws Michael.Daws at ucmp.umu.se
Mon Nov 13 10:40:06 EST 1995

I wish to immobilise a peptide on a column.  I think the easiest way to 
do this would be to biotinylate the peptide at the C-terminus and link 
it to streptavidin-agarose.
    a) Is this true?
    b) What is the easiest way to biotinylate specifically on the
       C-terminus of a peptide - do I link through a cysteine?
    c) How do I get rid of free biotin from a small peptide (approx.

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