How to deplete ATP in synaptosomes?

XZHENG at GAMMA.IS.TCU.EDU XZHENG at GAMMA.IS.TCU.EDU
Mon Nov 13 16:28:57 EST 1995


Hi, there!
     I am studying the effect of ATP on neurotransmitter release from rat
synaptosomes and trying to deplete ATP in synaptosomes. I tried some metabolic
inhibitors (4 ug/ml antimycin A, 30 mM deoxyglucose, 10 mM glucono-lactone), but it seemed that the depletion was not complete. What is the potency of antimycinA in inhibiting ATP generation? or any other inhibitors I can use? Please help!
and thank you in advance!
Alan Zheng
chemistry dept.
Texas Christian Univ.



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