In article <47jua7$ie7 at nuscc.nus.sg>, btccbh at leonis.nus.sg (Choo Boon Hwa Andre) writes:
> We have been unsuccessful in cloning DNA into pMal expression vectors.
> The few transformants that we obtained were either vector alone or
> rearranged vectors containing the insert. Has anyone observed instability
> of recombinant pMal vectors? We would appreciate if someone could respond
> to this query and share some ideas on how we might be able to overcome
> the problem.
Short answer? Choose another expression system. I'm afraid it's all trial and
Richard P. Grant MA DPhil rpgrant at molbiol.ox.ac.uk
Nuffield Department of Obstetrics and Gynaecology, University of Oxford.
"Winds light to variable"