pMAL Expression System

Mad Dan Eccles rpgrant at
Tue Nov 14 11:51:56 EST 1995

In article <47jua7$ie7 at>, btccbh at (Choo Boon Hwa Andre) writes:
> We have been unsuccessful in cloning DNA into pMal expression vectors.  
> The few transformants that we obtained were either vector alone or 
> rearranged vectors containing the insert. Has anyone observed instability 
> of recombinant pMal vectors? We would appreciate if someone could respond 

Oh yes.....

> to this query and share some ideas on how we might be able to overcome 
> the problem.

Short answer? Choose another expression system.  I'm afraid it's all trial and 
error :-(

Richard P. Grant MA DPhil      rpgrant at
Nuffield Department of Obstetrics and Gynaecology, University of Oxford.
"Winds light to variable"

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