pMAL Expression System

[Mad Dan Eccles]

In article <47jua7$ie7 at nuscc.nus.sg>, btccbh at leonis.nus.sg (Choo Boon Hwa Andre) writes:
> We have been unsuccessful in cloning DNA into pMal expression vectors.  
> The few transformants that we obtained were either vector alone or 
> rearranged vectors containing the insert. Has anyone observed instability 
> of recombinant pMal vectors? We would appreciate if someone could respond 

Oh yes.....

> to this query and share some ideas on how we might be able to overcome 
> the problem.

Short answer? Choose another expression system.  I'm afraid it's all trial and 
error :-(

Richard
-- 
Richard P. Grant MA DPhil      rpgrant at molbiol.ox.ac.uk
Nuffield Department of Obstetrics and Gynaecology, University of Oxford.
http://sable.ox.ac.uk/~lady0266
"Winds light to variable"