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Competent frozen yeast cells

Andrei Popov ANDREI.POPOV at bbsrc.ac.uk
Tue Nov 14 06:55:10 EST 1995

>I see that Stratagene are now supplying competent frozen yeast cells for use
>in the yeast two-hybrid system.  Has anyone used them and more interestingly
>does anyone know how they make them?  I have been looking for a protocol to
>produce competent yeast cells that can be stored at -70 similar to E.coli.
>Any hints or suggestions would be appreciated.

Spheroplasted yeast cells can be frozen with 7% DMSO.
Look for any decent yeast spheroplasting protocol
(e.g. which is used for YAC transformation)


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