Competent frozen yeast cells
ANDREI.POPOV at bbsrc.ac.uk
Tue Nov 14 06:55:10 EST 1995
>I see that Stratagene are now supplying competent frozen yeast cells for use
>in the yeast two-hybrid system. Has anyone used them and more interestingly
>does anyone know how they make them? I have been looking for a protocol to
>produce competent yeast cells that can be stored at -70 similar to E.coli.
>Any hints or suggestions would be appreciated.
Spheroplasted yeast cells can be frozen with 7% DMSO.
Look for any decent yeast spheroplasting protocol
(e.g. which is used for YAC transformation)
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