Transfection of suspension cells

[Stephen R. Lasky]

In article <melcher.5.001D3774 at mathematik.uni-marburg.de>,
melcher at mathematik.uni-marburg.de (Ralph Melcher) wrote:

> Hi guys,
> 
> I want to transfect U937 cells with plasmid-DNA.
> Does anyone have experiences with transfecion of human suspension
> cells ?

We use electroporation for U937 (our control cell model) and other myeloid
cells or B-cells.  It seems to work very well for both transient and
stable transfection.  If you would like more info, e-mail me at
Stephen_Lasky at Brown.EDU.

> I used Lipofection for adherent cells, but i do not known if 
> it works for suspension cells too.
> 

Lipofection didn't work in our hands the first time we tried it.  We never
tried it again because we already had electroporation working but I'm sure
that a little playing around would get it to work also.

SRLasky

-- 
Stephen R. Lasky Ph.D.
Section of Experimental Therapeutics
Roger Williams Medical Center
Providence, RI 02908
Phone: 401-456-5672      Fax: 401-456-6569
e:mail: Stephen_Lasky at brown.edu