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Competent frozen yeast cells

Zal Suldan Z-Suldan at ski.mskcc.org
Tue Nov 14 08:20:43 EST 1995

In article <SMER2.91.30A7F0CF at med.monash.edu.au>
SMER2 at med.monash.edu.au writes:

> I see that Stratagene are now supplying competent frozen yeast cells for use 
> in the yeast two-hybrid system.  Has anyone used them and more interestingly 
> does anyone know how they make them?  I have been looking for a protocol to 
> produce competent yeast cells that can be stored at -70 similar to E.coli.
> Any hints or suggestions would be appreciated.

Electrocompetent Yeast can be stored at -70. They do lose some
efficiency as compared to pre-freezing. but in general should be more
efficient than LiAc. The Red Book (Current Protocols) has a good
protocol for making them.

Good luck!


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