In article <SMER2.91.30A7F0CF at med.monash.edu.au>
SMER2 at med.monash.edu.au writes:
> I see that Stratagene are now supplying competent frozen yeast cells for use
> in the yeast two-hybrid system. Has anyone used them and more interestingly
> does anyone know how they make them? I have been looking for a protocol to
> produce competent yeast cells that can be stored at -70 similar to E.coli.
>> Any hints or suggestions would be appreciated.
Electrocompetent Yeast can be stored at -70. They do lose some
efficiency as compared to pre-freezing. but in general should be more
efficient than LiAc. The Red Book (Current Protocols) has a good
protocol for making them.