antibody affinity purification

[Zhonglin Chai]

Thomas Triolo wrote:
>
>     There is a very simple method in a manual called Guide to yeast
>genetics and molecular biology edited by Gutherie and Fink.  It involves
>running the antigen out on an SDS-PAGE gel, bloting it to
>nitrocellulose,and excising the band corresponding to the antigen.  You
>then incubate the band in the serum, wash it a few times and then elute
>it.
>
>     I hear it works very well.
>good luck

Hello Thomas,

        Do you have a protocol for this? Particularly how to elute the Ab
from the nitrocellulose? Do you have to dialyse, or how, to bring the Ab to
a right PH and buffer conditions?



ZhongLin Chai, PhD

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Department of Pathology and Immunology
Monash University Medical School
Alfred Hospital
Commercial Rd, Prahran, Victoria, AUSTRALIA
Telephone: (61 3) 9276 2698
           (61 3) 9388 1205 (home)
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email:     zchai at cobra.path.monash.edu.au
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