the function of -70 for X-ray film
dboyd at cc.umanitoba.ca
Tue Nov 14 17:10:43 EST 1995
In article <482t3c$ikj at uucp.intac.com>, jrt at intac.com (John R. Thompson) wrote:
> Stephen Lessnick <stevel at ucla.edu> wrote:
> >1) Why would one expose 35S sequencing gels at -70 degrees in the first
> >place since they don't possess enough energy to make the enhancing screen
> >worthwhile. On the other hand, soaking the gel in salicylate or PPO
> >would provide the enhancement effect, and then I believe it would make a
> >difference to expose the gel at -70.
> Use of a screen is not the only reason to expose at -70. The film
> emulsion itself retains an image better at -70. Thus irrespective of
> whether or not a screen is used, there is advantage to be gained by
> exposing at -70.
> John R. Thompson
> Merck Research Labs
I was just flipping through this article when I thought you might know
the effects of using "Amplify" from Biorad. I've been using it on my gels
(14C and 35S) and I think it does work although I have been getting a
"grainy" effect on my gels when I subject them to vaccuum and heat when
drying which totally distorts any bands that have been
stained(Coomassie).Lately, I haven't been using it and just leave the gels
on film longer. What a pain! Any suggestions or insight on Amplify?
EGreif at ccu.umanitoba.ca
Hey, hey, hey, hey! It was the DNA.
Hey, hey, hey, hey! That made me this way.
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