IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

the function of -70 for X-ray film

DAB dboyd at cc.umanitoba.ca
Tue Nov 14 17:10:43 EST 1995

In article <482t3c$ikj at uucp.intac.com>, jrt at intac.com (John R. Thompson) wrote:

> Stephen Lessnick <stevel at ucla.edu> wrote:
> >1)  Why would one expose 35S sequencing gels at -70 degrees in the first 
> >place since they don't possess enough energy to make the enhancing screen 
> >worthwhile.  On the other hand, soaking the gel in salicylate or PPO 
> >would provide the enhancement effect, and then I believe it would make a 
> >difference to expose the gel at -70.
> >
> Use of a screen is not the only reason to expose at -70.  The film
> emulsion itself retains an image better at -70.  Thus irrespective of
> whether or not a screen is used, there is advantage to be gained by
> exposing at -70.
> Regards,
> John R. Thompson
> Merck Research Labs

 I was just flipping through this article when I thought you might know
the effects of using "Amplify" from Biorad. I've been using it on my gels
(14C and 35S) and I think it does work although I  have been getting a
"grainy" effect on my gels when I subject them to vaccuum and heat when
drying which totally distorts any bands that have been
stained(Coomassie).Lately, I haven't been using it and just leave the gels
on film longer. What a pain!  Any suggestions or insight on Amplify?


EGreif at ccu.umanitoba.ca

Hey, hey, hey, hey! It was the DNA.
Hey, hey, hey, hey! That made me this way.

More information about the Methods mailing list

Send comments to us at biosci-help [At] net.bio.net