We are using non-denaturing polyacrylamide gels to resolve PCR-amplified
microsatellites. It seems to be working well except when we try to resolve
microsate;;ites in the 300-400 bp range using 7% acrylamide gels at 19:1
bis to acryl. We are running gels V16 aparatus overnight at 60 V. The
problem is that the marker and PCR products in this range are smeared. The
DNa seems to resolve below this range fine. We have tried changing and
remaking everything. Maybe the buffer is being depleted from such long runs?
nelsonj at pbs.dfo.ca