sequencing gels

[o. van-ham]

I had a similar prooblem some time ago.  It drove me nuts; I'd have a 
beautiful gel that would fall apart when I'd separate the plates.  The 
problem turned out to be that I wasn't letting the gel sit long enough 
after pouring.  I would pour the gel first thing in the morning, and run 
the gel early afternoon.  If you're not already doing so, I'd recommend 
pouring the gel at the end of the day, and running the gel in the 
morning.  This seems to give the gel enough time to adhere properly to 
the bind-silane coated plate.  Good luck.